MCP-2 Antibody [1.1_2D4-1A3] | XP-5730-M

MCP-2 Antibody [1.1_2D4-1A3] | XP-5730-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype.

Research Area: Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL) , a concentration of 0.8-1.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: The monoclonal antibody against human MCP-2 designated as VL-1 was purified from ascites fluid on a Protein A affinity column.

Clonality: Monoclonal

Clone: 1.1_2D4-1A3

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: CCL 8, HC14, Monocyte Chemotactic Protein 2, SCYA10, SCYA8, MCP2, C-C motif chemokine 8, MCP-2

User Note: Centrifuge vial prior to opening.

BACKGROUND: MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.