MEK1/2 Antibody | 8207

MEK1/2 Antibody | 8207 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (100%) , Chicken: (87%)

Immunogen: MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.
The immunogen is located within amino acids 240 - 290 of MEK1/2.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 43 kDa
Observed: 44 kDa

Validation: N/A

Isoform: N/A

Purification: MEK1/2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MEK1/2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Mitogen-activated protein kinase kinase 1, MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1, CFC3, Mitogen-activated protein kinase kinase 2, MAP2K2, MAPKK2, MEK2, MKK2, PRKMK2, CFC

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1) . MAP kinases, also known as extracellular signal-regulated kinases (ERKs) , act as an integration point for multiple biochemical signals (2) . MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2) .