MET, Active | 7771

MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kd () and 145 kd (). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2).

7771 | MET Active DataSheet

Biomolecule/Target: N/A

Synonyms: MET

Alternates names: MET, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Short name=SF receptor, Tyrosine-protein kinase Met

Taglines: A proto-oncogene

NCBI Gene ID #: 6348

NCBI Gene Symbol: CCL3

Gene Source: N/A

Accession #: P10147

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of MET was determined to be 46 nmol /min/mg as per activity assay protocol.

Biological activity: Activity was determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 74.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can be diluted into other aqueous buffers or store at 4°C for 1 week and –20°C for future use.

Amino acid sequence: N/A