Methylstat (hydrate) | B2836

Methylstat is a methyl ester prodrug that inhibits Jumonji C domain-containing histone demethylase (JMJD) in cells. Methylstat inhibits growth of JMJD2C-sensitive oesophageal carcinoma cell line KYSE150 cells with an IC₅₀ of approximately 5.1 μM, whereas the free acid of methylstat does not inhibit cell growth up to 100 µM. Methylstat induces hypermethylation, inhibits JMJD2C and recovers H3K9me3 levels in HeLa cells.

Methylstat is a methyl ester prodrug that inhibits Jumonji C domain-containing histone demethylase (JMJD) in cells. Methylstat inhibits growth of JMJD2C-sensitive oesophageal carcinoma cell line KYSE150 cells with an IC₅₀ of approximately 5.1 μM, whereas the free acid of methylstat does not inhibit cell growth up to 100 µM. Methylstat induces hypermethylation, inhibits JMJD2C and recovers H3K9me3 levels in HeLa cells.

B2836 | Methylstat (hydrate) DataSheet

Alternate Name/Synonyms: 4-[hydroxy[4-[[[4-[[[(1-naphthalenylamino)carbonyl]oxy]methyl]phenyl]methyl]amino]butyl]amino]-4-oxo-2E-butenoic acid, methyl ester, monohydrate; (E)-methyl 4-(hydroxy(4-((4-(((naphthalen-1-ylcarbamoyl)oxy)methyl)benzyl)amino)butyl)amino)-4-oxobut-2-enoate hydrate; Histone Lysine Demethylase Inhibitor VII; JHDM Inhibitor IV

Appearance: Crystalline solid

Formulation:

CAS Number: n/a

Structure Available?: true

Peptide sequence:

Salt Form: false

Molecular Formula: C₂₈H₃₁N₃O₆.H₂O

Molecular Weight: 523.58

Cell-Permeable?: True

Purity: ≥98%

Solubilities: ~25 mg/ml in DMSO and DMF in 0

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A prodrug of a Jumonji C domain-containing histone demethylase (JMJD)

MDL Number:

PubChem CID:

SMILES: O=C(OC)/C=C/C(N(O)CCCCNCC1=CC=C(COC(NC2=C3C=CC=CC3=CC=C2)=O)C=C1)=O.[H]O[H]

InChi: InChI=1S/C28H31N3O6.H2O/c1-36-27(33)16-15-26(32)31(35)18-5-4-17-29-19-21-11-13-22(14-12-21)20-37-28(34)30-25-10-6-8-23-7-2-3-9-24(23)25;/h2-3,6-16,29,35H,4-5,17-20H2,1H3,(H,30,34);1H2/b16-15+;

InChi Key: GLUJKORUIWJKCZ-GEEYTBSJSA-N