METTL14 Antibody (N-Term) [APR33651G]

METTL14 Antibody (N-Term) [APR33651G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This METTL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the human region of human METTL14.

Cellular Localisation: Nucleus

Molecular Weight: 52150

Clone: Polyclonal

Gene Name: METTL14 (HGNC:29330)

Gene ID: 57721

Function: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N (6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:29348140, PubMed:27373337, PubMed:27281194) . In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:29348140) . N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671) . M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity) . In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency- promoting transcripts results in transcript destabilization (By similarity) . M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity) . M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity) .

Summary: N/A

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: IF, WB, IHC-P

Dilution: IHC-P--1:250 WB--1:2000

Synonyms: N6-adenosine-methyltransferase subunit METTL14, 2.1.1.62, Methyltransferase-like protein 14, METTL14, KIAA1627