MFN1 Antibody | 7861

MFN1 Antibody | 7861 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: MFN1 antibody was raised against a 17 amino acid peptide near the amino terminus of human MFN1.
The immunogen is located within amino acids 20 - 70 of MFN1.

Research Area: Obesity, Apoptosis

Tested Application: E, WB, ICC

Application: MFN1 antibody can be used for detection of MFN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: MFN1 antibody is human, mouse and rat reactive. MFN1 antibody is predicted to not cross-react with MFN2.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 82 kDa
Observed: 83 kDa

Validation: N/A

Isoform: N/A

Purification: MFN1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MFN1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MFN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: MFN1 Antibody: hfzo1, hfzo2, Mitofusin-1, Fzo homolog

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1, 2) . MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development (3) . When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane (4) .