MFN2 Antibody | 7863

MFN2 Antibody | 7863 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2.
The immunogen is located within amino acids 250 - 300 of MFN2.

Research Area: Obesity, Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Specificiy: MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 83 kDa
Observed: 90 kDa

Validation: N/A

Isoform: N/A

Purification: MFN2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MFN2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MFN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: MFN2 Antibody: HSG, MARF, CMT2A, CPRP1, CMT2A2, KIAA0214, Mitofusin-2, Transmembrane GTPase MFN2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1, 2) . MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3, 4) . Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5) .