MGMT (C5) polyclonal Antibody | BS2540

MGMT (C5) polyclonal Antibody | BS2540 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MGMT (C5) polyclonal Antibody detects endogenous levels of MGMT protein.

Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: MGMT; EC 2.1.1.63; Methylated-DNA--protein-cysteine methyltransferase

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human MGMT.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: