MGMT polyclonal Antibody | BS90854

MGMT polyclonal Antibody | BS90854 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC

Application Range: WB:1:1,000-1:2,000 IHC:1:50-1:200

Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MGMT polyclonal Antibody detects endogenous levels of MGMT protein.

Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: MGMT, 6 O methylguanine DNA methyltransferase, 6-O-methylguanine-DNA methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA protein cysteine methyltransferase, Methylated-DNAprotein-cysteine methyltransferase, Methylguanine DNA methyltransferase, MGC107020, MGMT, MGMT, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: