MIA-2, human recombinant | 4888

MIA2 performs as a tumour suppressor in hepatocellular carcinoma. MIA2 is a novel acute phase protein which its expression is found in the liver and reacts to liver damage in chronic liver diseases and is considerably higher in severe fibrosis patients . MIA2 is induced by IL6, TGF- and conditioned medium from activated hepatic stellate cells. MIA2 is is mainly expressed in hepatocytes. MIA2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain consisting of 101 amino having a total molecular mass of 11.5 kDa. The MIA2 is purified by proprietary chromatographic techniques.

4888 | MIA-2 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Melanoma inhibitory activity protein 2

Alternates names: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta 3.

Taglines: Potential role in the pathophysiology of liver disease

NCBI Gene ID #: 7043

NCBI Gene Symbol: TGFB3

Gene Source: Human

Accession #: P10600

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Results: 0.1 to 0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25 kDa, homodimer, nonglycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered white powder lyophilized from a solution containing 20 mM phosphate buffer pH 7.4 and 150 mM NaCl.

Reconstitution Instructions: Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A