Description
MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extraCellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.
4887 | MIA human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA
Alternates names: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta 3.
Taglines: An inhibitor of in vitro growth of malignant melanoma cells
NCBI Gene ID #: 7043
NCBI Gene Symbol: TGFB3
Gene Source: Human
Accession #: P10600
Recombinant: Yes
Source: Human 293 cell expressed
Purity by SDS-PAGEs: > 95%
Assay: SDS-PAGE
Purity: 98%
Assay #2: HPLC
Endotoxin Level: < 1.0 EU per 1 g of protein
Activity (Specifications/test method): N/A
Biological activity: ED is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
Results: 0.1 to 0.5 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 25 kDa, homodimer, nonglycosylated
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered and lyophilized with no additives
Reconstitution Instructions: Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin.
Amino acid sequence: N/A