MIA, human recombinant | 4887

MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extraCellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.

4887 | MIA human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA

Alternates names: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta 3.

Taglines: An inhibitor of in vitro growth of malignant melanoma cells

NCBI Gene ID #: 7043

NCBI Gene Symbol: TGFB3

Gene Source: Human

Accession #: P10600

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Results: 0.1 to 0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25 kDa, homodimer, nonglycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives

Reconstitution Instructions: Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A