MIB1 Antibody | 7781

MIB1 Antibody | 7781 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1.
The immunogen is located within amino acids 920 - 970 of MIB1.

Research Area: Homeostasis, Apoptosis

Tested Application: E, WB, ICC, IF

Application: MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 89, 102, 111 kDa
Observed: 103 kDa

Validation: N/A

Isoform: N/A

Purification: MIB1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MIB1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MIB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: MIB1 Antibody: MIB, DIP1, ZZZ6, DIP-1, LVNC7, ZZANK2, KIAA1323, E3 ubiquitin-protein ligase MIB1, DAPK-interacting protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1) . MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1) . It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2) .