Midkine, murine recombinant | 7158

Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorigenesis. Recombinant murine Midkine is a 13.3 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds.

7158 | Midkine murine recombinant DataSheet

Biomolecule/Target: Midkine

Synonyms: MK, NEGF-2

Alternates names: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Taglines: Plays an important regulatory role in epithelial-mesenchymal interactions

NCBI Gene ID #: 7124

NCBI Gene Symbol: TNF

Gene Source: Human

Accession #: P01375

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically < 0.9 ng/mL. The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.

Results: < 0.9 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 kDa, non-disulfide bonded homotrimer, glycosylated

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.4 x PBS, pH 7.4.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A