MIF, human recombinant | 4984

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa.

4984 | MIF human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.

Alternates names: Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Taglines: A protein which triggesr an acute immune response

NCBI Gene ID #: 7066

NCBI Gene Symbol: THPO

Gene Source: Human

Accession #: P40225

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.5 to 5 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human M07e cells

Results: 0.5 to 5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80 to 85 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: MIF-Protein was lyophilized from 10 mM sodium phosphate buffer pH-7.5.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A