MIG Antibody [1.1_2D3-2H3] | XP-5721-M

MIG Antibody [1.1_2D3-2H3] | XP-5721-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hMIG as the immunizing antigen.

Research Area: Chemokines & Cytokines, Signal Transduction, Homeostasis

Tested Application: E, WB

Application: ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Sandwich:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.

Clonality: Monoclonal

Clone: 1.1_2D3-2H3

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: MIG lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: monokine induced by interferon-gamma, CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, HuMIG, Humig, crg-10

User Note: Centrifuge vial prior to opening.

BACKGROUND: MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor.