MIP-3 alpha Antibody [G3_4G10.3] | 38-236

MIP-3 alpha Antibody [G3_4G10.3] | 38-236 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: MIP-3 alpha antibody is produced in mice using highly pure (>98%) recombinant human MIP- 3alpha as the immunizing antigen.

Research Area: Immunology, Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL) , a concentration of 3.0-5.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Clonality: Monoclonal

Clone: G3_4G10.3

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: MIP-3 alpha lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: Macrophage Inflammatory Protein 3 alpha, SCYA20, Small inducible cytokine A20, LARC, Liver and activation regulated chemokine, exodus-1, MIP3A, C-C motif chemokine 20, Beta-chemokine exodus-1, MIP-3-alpha, CKb4, ST38, MIP-3a

User Note: Centrifuge vial prior to opening.

BACKGROUND: Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It binds to CCR6.

Was product number XP-5713-M