MLH1 Antibody (Center) (Ascites) [AMM02440G]

MLH1 Antibody (Center) (Ascites) [AMM02440G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This MLH1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 458-489 amino acids from the Central region of human MLH1.

Cellular Localisation: Nucleus. Chromosome Note=Recruited to chromatin in a MCM9-dependent manner

Molecular Weight: 84601

Clone: Monoclonal

Gene Name: MLH1

Gene ID: 4292

Function: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR) . DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Summary: Tissue Location: Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart

Form: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:500~1000

Synonyms: DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1, COCA2