MMP-14, Active, Human Recombinant | 8009

The Matrix metalloproteinase-14 (MMP-14) belongs to a family of zinc-dependent endopeptidases. The ability of MMP-14 to degrade type I collagen, and activate pro-MMP-2 and pro-MMP-9 makes it a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. MMP-14 is structurally a multi-domain metalloenzyme, composed of a prodomain, a metal-binding catalytic domain, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail. The catalytic, and hinge domains of MT1-MMP were expressed in Escherichia coli and the active form was generated by successive autoproteolysis of the N- and C-terminal sites during refolding.

8009 | MMP-14 Active Human Recombinant DataSheet

Biomolecule/Target: MMP-14

Synonyms: Matrix metalloproteinase-14, MT1-MMP

Alternates names: Protein A

Taglines: >90% Pure, Active, Human Recombinant MMP-14

NCBI Gene ID #: 3919448

NCBI Gene Symbol: N/A

Gene Source: Staphylococcus aureus

Accession #: YP_498670

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 µmol of Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.

Molecular Weight: 39.21 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from propriety buffer

Reconstitution Instructions: Reconstitute in HO

Amino acid sequence: N/A