Mouse 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit | AE60147MO

Mouse 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit | AE60147MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: PFKFB2

Alternative Name: DKFZp781D2217; MGC138308; MGC138310; PFK-2/FBPase-2; 6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 2|6PF-2-K/Fru-2;6-P2ASE heart-type isozyme|PFKFB; cardiac|fructose-2;6-bisphosphatase; cardi

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.137 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤7.5%

Recovery: 1

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PFKFB2 in samples. An antibody specific for PFKFB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKFB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKFB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKFB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 is involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate, and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of fructose-2, 6-bisphosphate. This protein regulates fructose-2, 6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2, 6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .