Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit | KTE70534

Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit | KTE70534 | Gentaur UK, US & Europe Distribution

Application: This Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit employs a two-site sandwich ELISA to quantitate ACACA in samples. An antibody specific for ACACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACACA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Acetyl-CoA carboxylase 1 microplate
• Mouse Acetyl-CoA carboxylase 1 standard
• Mouse Acetyl-CoA carboxylase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACACA. No significant cross-reactivity or interference between Mouse ACACA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT) . ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation.

Alternative Names: ACACA; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha

Search name: ACACA; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha

Tag: ACACA