Mouse ADH1 (Alcohol Dehydrogenase 1) ELISA Kit | ELK3002

Mouse ADH1 (Alcohol Dehydrogenase 1) ELISA Kit | ELK3002 | Gentaur UK, US & Europe Distribution

Alternative Names: ADH1A; Alcohol Dehydrogenase 1A (Class I) Alpha Polypeptide; Alcohol dehydrogenase subunit alpha

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.133 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the OD of the samples to the standard curve.