Mouse AhR (Aryl Hydrocarbon Receptor) ELISA Kit | E-EL-M0156

Mouse AhR (Aryl Hydrocarbon Receptor) ELISA Kit | E-EL-M0156 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 0.23ng/mL

Detection Range: 0.39~25ng/mL

UniProt ID: P30561 

Target Name: AhR

Target Synonym: bHLHe76

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AhR. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse AhR. You can calculate the concentration of Mouse AhR in the samples by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Mouse AhR in samples. No significant cross-reactivity or interference between Mouse AhR and analogues was observed.

Research Areas: Cancer, Epigenetics and Nuclear Signaling

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.