Mouse Anti-Human EpCAM Antibody | 101-M389

Mouse Anti-Human EpCAM Antibody | 101-M389 | Gentaur UK, US & Europe Distribution

Species: Anti-Human

Host / biotech: Mouse

Comment: N/A

Label: N/A

Clone / Antibody feature: (#8D36)

Subcategory: Monoclonal Antibody

Category: Antibody

Synonyms: EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Isotype: IgG2

Application: WB, IHC

Detection Range: N/A

Species Reactivity/Cross reactivity: Human

Antigen: recombinant human EpCAM

Description: Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA7332, 171A, and TROP1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal growth factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with Trop2/ EGP1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudostratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuroendocrine tissue, or lymphoid tissue. EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration. EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia. EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule. Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers. During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association α-actinin.

Purity Confirmation: N/A

Endotoxin: N/A

Formulation: lyophilized

Storage Handling Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Reconstituation: Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Molecular Weight: N/A

Lenght (aa): N/A

Protein Sequence: N/A

NCBI Gene ID: 4072