MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) INTEGRASE (2)

Mouse anti HIV-1 Integrase (2) antibody is specific for HIV-I integrase protein and has been developed for use in ELISA, Western blot and immunofluorescence.

PRODUCT DETAILS – MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) INTEGRASE (2)

• Mouse anti HIV-1 Integrase (Clone 2). Mouse IgG1,κ.
• Specific for HIV-I integrase protein. No cross-reactivity with non-HIV-1 integrase proteins has been observed.
• Purified from supernatants of hybridoma cell cultures by affinity chromatography.
• Presented in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.
• For use in ELISA, western blotting and immunofluorescence.

BACKGROUND

Human immunodeficiency virus (HIV) is a retrovirus (genus Lentivirus) with a single-stranded, positive-sense RNA genome. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase allowing the genome to be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated to infect other cells. Infection with HIV leads to a condition in which the immune system begins to fail, leading to opportunistic infections.

Integration of viral DNA into a chromosome of the host cell is an essential step in the retroviral life cycle. This process is catalyzed by the viral enzyme integrase (IN) through 3 steps: first step, two nucleotides are removed from the 39 ends of the viral DNA (39-end processing); second step, the recessed 39 ends of the viral DNA are then joined to 59 staggered sites in the target DNA in a concerted cleavage and ligation reaction (DNA joining); last step, integration is completed by repair of the short gaps flanking the viral DNA intermediate and subsequent joining of the 59 ends of viral DNA to the target DNA.

REFERENCES

• Chiu and Davies (2004). Structure and function of HIV-1 integrase. Curr Top Med Chem. 4(9):965-77.