Mouse Aspartyl-tRNA synthetase, cytoplasmic (DARS) ELISA Kit | KTE71334

Mouse Aspartyl-tRNA synthetase, cytoplasmic (DARS) ELISA Kit | KTE71334 | Gentaur UK, US & Europe Distribution

Application: This Mouse Aspartyl-tRNA synthetase, cytoplasmic (DARS) ELISA Kit employs a two-site sandwich ELISA to quantitate DARS in samples. An antibody specific for DARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DARS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DARS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Aspartyl-tRNA synthetase, cytoplasmic microplate
• Mouse Aspartyl-tRNA synthetase, cytoplasmic standard
• Mouse Aspartyl-tRNA synthetase, cytoplasmic detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Aspartyl-tRNA synthetase, cytoplasmic (DARS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DARS. No significant cross-reactivity or interference between Mouse DARS and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Aspartyl-tRNA synthetase, cytoplasmic (DARS) encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA (Asp) . Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.

Alternative Names: DARS; DKFZp781B11202; MGC111579; aspartate tRNA ligase 1; cytoplasmic; cell proliferation-inducing protein 40

Search name: DARS; DKFZp781B11202; MGC111579; aspartate tRNA ligase 1; cytoplasmic; cell proliferation-inducing protein 40

Tag: DARS