Mouse BLOC1S1 (Biogenesis of lysosome-related organelles complex 1 subunit 1) ELISA Kit | ELK8891

Mouse BLOC1S1 (Biogenesis of lysosome-related organelles complex 1 subunit 1) ELISA Kit | ELK8891 | Gentaur UK, US & Europe Distribution

Alternative Names: BLOC1S1 ; BLOS1 ; GCN5L1 ; RT14Biogenesis of lysosome-related organelles complex 1 subunit 1 ; BLOC-1 subunit 1 ; GCN5-like protein 1 ; Protein RT14

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) in the samples is then determined by comparing the OD of the samples to the standard curve.