Mouse C5aR1 (Complement Component 5a Receptor 1) ELISA Kit | ELK4243

Mouse C5aR1 (Complement Component 5a Receptor 1) ELISA Kit | ELK4243 | Gentaur UK, US & Europe Distribution

Alternative Names: CD88; C5A; C5-AR1; C5R1; C5a anaphylatoxin chemotactic receptor 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a Receptor 1 (C5aR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Component 5a Receptor 1 (C5aR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5a Receptor 1 (C5aR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5a Receptor 1 (C5aR1) in the samples is then determined by comparing the OD of the samples to the standard curve.