Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit | KTE71099

Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit | KTE71099 | Gentaur UK, US & Europe Distribution

Application: This Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit employs a two-site sandwich ELISA to quantitate MCAM in samples. An antibody specific for MCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Cell surface glycoprotein MUC18 microplate
• Mouse Cell surface glycoprotein MUC18 standard
• Mouse Cell surface glycoprotein MUC18 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCAM. No significant cross-reactivity or interference between Mouse MCAM and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CD146 (cluster of differentiation 146) is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.

Alternative Names: MCAM; CD146; MUC18; Gicerin; melanoma adhesion molecule

Search name: MCAM; CD146; MUC18; Gicerin; melanoma adhesion molecule

Tag: MCAM