Mouse CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit | KTE71436

Mouse CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit | KTE71436 | Gentaur UK, US & Europe Distribution

Application: This Mouse CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKG2 in samples. An antibody specific for PRKG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse CGMP-dependent protein kinase 2 microplate
• Mouse CGMP-dependent protein kinase 2 standard
• Mouse CGMP-dependent protein kinase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse CGMP-dependent protein kinase 2 (PRKG2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRKG2. No significant cross-reactivity or interference between Mouse PRKG2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Pfeifer et al. (1996) noted that there are 2 forms of cGMP-dependent protein kinase (cGK), types I and II, which are encoded by distinct genes. Smooth muscle, platelets, and cerebellum contain high concentrations of cGKI, whereas cGKII is highly concentrated in brain, lung, and intestinal mucosa. Orstavik et al. (1996) cloned a human cDNA encoding type II cGK by using the mouse type II cGK cDNA to probe a cerebellum cDNA library. The 762-amino acid human type II cGK protein is 96% identical to the mouse and rat type II cGK proteins. Human type II cGK is expressed as a 6-kb mRNA in prostate, small intestine, and colon and as a 4.4-kb mRNA in thymus and prostate.

Alternative Names: PRKG2; PRKGR2; cGKII; OTTHUMP00000160807

Search name: PRKG2; PRKGR2; cGKII; OTTHUMP00000160807

Tag: PRKG2