Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit | AE58990MO

Mouse Crystallin, beta A1 (CRYBA1) ELISA Kit | AE58990MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: CRYBA1

Alternative Name: CRYB1; crystallin; beta A3|eye lens structural protein

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.118 ng/mL

Intra-Assay: ≤5.6%

Inter-Assay: ≤9.2%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CRYBA1 in samples. An antibody specific for CRYBA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCRYBA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRYBA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRYBA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group) . Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. CRYbA1, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .