Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit | KTE71261

Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit | KTE71261 | Gentaur UK, US & Europe Distribution

Application: This Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit employs a two-site sandwich ELISA to quantitate DLD in samples. An antibody specific for DLD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Dihydrolipoyl dehydrogenase microplate
• Mouse Dihydrolipoyl dehydrogenase standard
• Mouse Dihydrolipoyl dehydrogenase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Dihydrolipoyl dehydrogenase (DLD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLD. No significant cross-reactivity or interference between Mouse DLD and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The dihydrolipoate, still bound to a lysine residue of the complex, then migrates to the dihydrolipoyl dehydrogenase (E3) active site where it undergoes a flavin-mediated oxidation, identical in chemistry to disulfide isomerase. First, FAD oxidizes dihydrolipoate back to its lipoate resting state, producing FADH2. Dihydrolipoamide dehydrogenase is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Alternative Names: DLD; tcag7.39; DLDH; E3; GCSL; LAD; PHE3; E3 component of pyruvate dehydrogenase complex; 2-oxo-glutarate complex; branched chain keto acid dehydrogenase complex; OTTHUMP00000206749; diaphorase; dihydrolipo

Search name: DLD; tcag7.39; DLDH; E3; GCSL; LAD; PHE3; E3 component of pyruvate dehydrogenase complex; 2-oxo-glutarate complex; branched chain keto acid dehydrogenase complex; OTTHUMP00000206749; diaphorase; dihydrolipo

Tag: DLD