Mouse Disks large-associated protein 1 (DLGAP1) ELISA Kit | KTE71255

Mouse Disks large-associated protein 1 (DLGAP1) ELISA Kit | KTE71255 | Gentaur UK, US & Europe Distribution

Application: This Mouse Disks large-associated protein 1 (DLGAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP1 in samples. An antibody specific for DLGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Disks large-associated protein 1 microplate
• Mouse Disks large-associated protein 1 standard
• Mouse Disks large-associated protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Disks large-associated protein 1 (DLGAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DLGAP1. No significant cross-reactivity or interference between Mouse DLGAP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DLGAP1encodes the protein called guanylate kinase-associated protein (GKAP) . GKAP binds to the SHANK and PSD-95 proteins fascilitating the assembly of the post synaptic density of neurons. Sequence analysis predicted that the 627-amino acid hydrophilic protein, which is 98% identical to the rat sequence, contains five 14-amino acid repeats in its N terminus. Binding analysis determined that the N-terminal region of DLGAP1 interacts with intact GK domains of all members of the DLG family, which share approximately 75% homology in the GK domain; DLGAP1 was found to interact less well with more distantly related MAGUKs. Immunoblot analysis showed expression of 95- and 130-kD DLGAP1 proteins in membranes from different regions of rat brain.

Alternative Names: DLGAP1; DAP-1; DAP-1-ALPHA; DAP-1-BETA; GKAP; MGC88156; SAPAP1; hGKAP; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; discs large homolog-associated protein 1; disks large-associated protei

Search name: DLGAP1; DAP-1; DAP-1-ALPHA; DAP-1-BETA; GKAP; MGC88156; SAPAP1; hGKAP; PSD-95/SAP90 binding protein 1; SAP90/PSD-95-associated protein 1; discs large homolog-associated protein 1; disks large-associated protei

Tag: DLGAP1