Mouse Eif2ak4 Antibody (C-term) [APC00084G]

Mouse Eif2ak4 Antibody (C-term) [APC00084G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Eif2ak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1477-1504 amino acids from the C-terminal region of mouse Eif2ak4.

Cellular Localisation: Cytoplasm.

Molecular Weight: 186487

Clone: Polyclonal

Gene Name: Eif2ak4 {ECO:0000312|MGI:MGI:1353427}

Gene ID: 27103

Function: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367) . Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367) . EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367) . Binds uncharged tRNAs (By similarity) . Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:16176978, PubMed:26102367) . Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (PubMed:16121183) . Plays a role in neurite outgrowth inhibition (PubMed:23447528) . Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition (PubMed:16054071, PubMed:15774759) . Plays a proapoptotic role in response to glucose deprivation (PubMed:20660158) . Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress- activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (PubMed:12176355) .

Summary: Tissue Location: Expressed in liver (PubMed:10504407) . Expressed predominantly in the hippocampal CA1 region and the dentate gyrus, and to a lesser degree in CA3 (at protein level) (PubMed:16121183) Expressed in liver, lung, brain, kidney, skeletal muscle and testis (PubMed:10504407, PubMed:10655230) . Expressed weakly in heart and spleen (PubMed:10655230) . Expressed in the hippocampal CA1 and CA3 regions, the dentate gyrus and cerebellum (PubMed:16121183) . Isoform 1 is widely expressed (PubMed:12215525) . Isoform 1 is expressed in brain, liver, skeletal muscle and testis (PubMed:10655230) . Isoform 3 is expressed in lung, brain, testis, prostate and choroid plexus (PubMed:12215525) . Isoform 4 is expressed in muscle, lung, kidney, brain, testis and prostate (PubMed:10655230, PubMed:12215525)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein, mGCN2, Eif2ak4, Gcn2, Kiaa1338