Mouse Ern1 Antibody (C-term) [APR03932G]

Mouse Ern1 Antibody (C-term) [APR03932G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This Mouse Ern1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 827-855 amino acids from the C-terminal region of mouse Ern1.

Cellular Localisation: Endoplasmic reticulum membrane; Single-pass type I membrane protein

Molecular Weight: 110185

Clone: Polyclonal

Gene Name: Ern1 {ECO:0000312|MGI:MGI:1930134}

Gene ID: 78943

Function: Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11850408, PubMed:25164867) . In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:25164867) . The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11850408, PubMed:25164867) . The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11850408, PubMed:25164867) . Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi- independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (By similarity) .

Summary: Tissue Location: Ubiquitously expressed. High levels in thymus, liver and lung. In the brain, preferentially expressed in cortical, hippocampal and olfactory neurons.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, Ern1 {ECO:0000312|MGI:MGI:1930134}