Mouse Factor-related Apoptosis (FAS) ELISA Kit | KTE71551

Mouse Factor-related Apoptosis (FAS) ELISA Kit | KTE71551 | Gentaur UK, US & Europe Distribution

Application: This Mouse Factor-related Apoptosis (FAS) ELISA Kit employs a two-site sandwich ELISA to quantitate FAS in samples. An antibody specific for FAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Factor-related Apoptosis microplate
• Mouse Factor-related Apoptosis standard
• Mouse Factor-related Apoptosis detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Factor-related Apoptosis (FAS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FAS. No significant cross-reactivity or interference between Mouse FAS and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Fas is a glycoprotein with a mass estimated at 43 to 48 kDa . Fas is a member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF), and it shares a cytoplasmic motif with TNF RI, referred to as the death domain, that binds cytoplasm signaling molecules to trigger the cytoplasmic apoptotic signal . Fas is expressed to a large extent on activated T and B lymphocytes, and on malignant lymphoid cells. To a lesser extent, Fas is expressed on cells from liver, heart, kidney, ovaries, and on many other malignant cells. Yan et al. (2005) noted that the TNFRSF6 gene contains 9 exons. They identified an antisense transcript SAF within the 12.1-kb intron 1 that is transcribed in the opposite direction as the TNFRSF6 gene.

Alternative Names: FAS; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; APO-1 cell surface antigen; CD95 antigen; Fas AMA; Fas antigen; apoptosis antigen 1; tumor necrosis factor receptor superfamily; member 6

Search name: FAS; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; APO-1 cell surface antigen; CD95 antigen; Fas AMA; Fas antigen; apoptosis antigen 1; tumor necrosis factor receptor superfamily; member 6

Tag: FAS