Mouse FGFR3 (Fibroblast Growth Factor Receptor 3) ELISA Kit | ELK4327

Mouse FGFR3 (Fibroblast Growth Factor Receptor 3) ELISA Kit | ELK4327 | Gentaur UK, US & Europe Distribution

Alternative Names: CD333; ACH; CEK2; JTK4; Achondroplasia; Thanatophoric Dwarfism

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 3 (FGFR3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor Receptor 3 (FGFR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor Receptor 3 (FGFR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor Receptor 3 (FGFR3) in the samples is then determined by comparing the OD of the samples to the standard curve.