Mouse Free fatty acid receptor 2 (FFAR2) ELISA Kit | KTE71390

Mouse Free fatty acid receptor 2 (FFAR2) ELISA Kit | KTE71390 | Gentaur UK, US & Europe Distribution

Application: This Mouse Free fatty acid receptor 2 (FFAR2) ELISA Kit employs a two-site sandwich ELISA to quantitate FFAR2 in samples. An antibody specific for FFAR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFFAR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FFAR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FFAR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Free fatty acid receptor 2 microplate
• Mouse Free fatty acid receptor 2 standard
• Mouse Free fatty acid receptor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Free fatty acid receptor 2 (FFAR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FFAR2. No significant cross-reactivity or interference between Mouse FFAR2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The physiologic responses to galanin (GAL) are mediated through specific membrane receptors called GALRs, which are members of the G protein-coupled receptor (GPCR) family. One product contained a segment showing 100% homology to a portion of the 3-prime region of the human CD22 gene . The authors identified a PAC clone in the sequence databases that overlaps this region and contains a novel putative GPCR gene, GPR43. The intronless GPR43 gene is located approximately 77 kb downstream of the GPR42 gene . GPR43 encodes a deduced 330-amino acid protein with 7 transmembrane domains. The GPR43 protein shares 28% amino acid identity with GPR40and little similarity with GALRs.

Alternative Names: FFAR2; FFA2R; GPR43; G protein-coupled receptor 43; free fatty acid activated receptor 2

Search name: FFAR2; FFA2R; GPR43; G protein-coupled receptor 43; free fatty acid activated receptor 2

Tag: FFAR2