Mouse Fructose-1, 6-bisphosphatase isozyme 2 (FBP2) ELISA Kit | KTE71492

Mouse Fructose-1, 6-bisphosphatase isozyme 2 (FBP2) ELISA Kit | KTE71492 | Gentaur UK, US & Europe Distribution

Application: This Mouse Fructose-1, 6-bisphosphatase isozyme 2 (FBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate FBP2 in samples. An antibody specific for FBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Fructose-1, 6-bisphosphatase isozyme 2 microplate
• Mouse Fructose-1, 6-bisphosphatase isozyme 2 standard
• Mouse Fructose-1, 6-bisphosphatase isozyme 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Fructose-1, 6-bisphosphatase isozyme 2 (FBP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FBP2. No significant cross-reactivity or interference between Mouse FBP2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Insulin-like growth factor binding protein-2 (IGFBP-2) is one of six characterized IGFBPs which specifically bind and modulate the actions of IGF-I and IGF-II. The binding affinity of IGFBP-2 for IGF-II is estimated to be significantly higher than for IGF-I, suggesting a role in regulation of IGF-II action. Studies in the fetal sheep indicate that IGFBP-2 is produced in many tissues, apparently coincident with IGF-II expression.

Alternative Names: FBP2; MGC142192; D-fructose-1;6-bisphosphate 1-phosphohydrolase; FBPase; fructose-1;6-bisphosphatase isozyme 2; hexosediphosphatase; muscle fructose-bisphosphatase

Search name: FBP2; MGC142192; D-fructose-1;6-bisphosphate 1-phosphohydrolase; FBPase; fructose-1;6-bisphosphatase isozyme 2; hexosediphosphatase; muscle fructose-bisphosphatase

Tag: FBP2