Mouse GAL8 (Galectin 8) ELISA Kit | ELK2897

Mouse GAL8 (Galectin 8) ELISA Kit | ELK2897 | Gentaur UK, US & Europe Distribution

Alternative Names: LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin, Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 8 (GAL8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galectin 8 (GAL8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 8 (GAL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 8 (GAL8) in the samples is then determined by comparing the OD of the samples to the standard curve.