Mouse GCP2 (Granulocyte Chemotactic Protein 2) ELISA Kit | ELK4004

Mouse GCP2 (Granulocyte Chemotactic Protein 2) ELISA Kit | ELK4004 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL6; SCYB6; GCP2; CKA-3; Chemokine (C-X-C-Motif) ligand 6; Small Inducible Cytokine Subfamily B (Cys-X-Cys) Member 6; Chemokine alpha 3; Small-inducible cytokine B6

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.78 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulocyte Chemotactic Protein 2 (GCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granulocyte Chemotactic Protein 2 (GCP2) in the samples is then determined by comparing the OD of the samples to the standard curve.