Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit | KTE71487

Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit | KTE71487 | Gentaur UK, US & Europe Distribution

Application: This Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit employs a two-site sandwich ELISA to quantitate GAB2 in samples. An antibody specific for GAB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse GRB2-associated-binding protein 2 microplate
• Mouse GRB2-associated-binding protein 2 standard
• Mouse GRB2-associated-binding protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GAB2. No significant cross-reactivity or interference between Mouse GAB2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: GAB2 is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. In contrast to the reciprocal effects of GAB1 and GAB2 in mediating ELK1 induction, these 2 molecules have a similar function in extracellular signal-regulated kinase activation induced by either oncogenic Ras or growth factor stimulation.

Alternative Names: GAB2; KIAA0571; Grb2-associated binder 2; growth factor receptor bound protein 2-associated protein 2

Search name: GAB2; KIAA0571; Grb2-associated binder 2; growth factor receptor bound protein 2-associated protein 2

Tag: GAB2