Mouse Group XIIB secretory phospholipase A2-like protein (PLA2G12B) ELISA Kit | KTE70743

Mouse Group XIIB secretory phospholipase A2-like protein (PLA2G12B) ELISA Kit | KTE70743 | Gentaur UK, US & Europe Distribution

Application: This Mouse Group XIIB secretory phospholipase A2-like protein (PLA2G12B) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G12B in samples. An antibody specific for PLA2G12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G12B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Group XIIB secretory phospholipase A2-like protein microplate
• Mouse Group XIIB secretory phospholipase A2-like protein standard
• Mouse Group XIIB secretory phospholipase A2-like protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Group XIIB secretory phospholipase A2-like protein (PLA2G12B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLA2G12B. No significant cross-reactivity or interference between Mouse PLA2G12B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The predicted 195-amino acid human protein shares 90% identity with the mouse protein and 40% identity with human PLA2G12A. It contains a 19-amino acid signal peptide and 14 cysteines, but it lacks N-glycosylation sites and has a leucine in place of the canonical histidine in its active site. PLA2G12B is conserved in vertebrates, but not in invertebrates. SDS-PAGE revealed a 20-kD PLA2G12B protein, consistent with the calculated molecular mass of the mature protein. Northern blot analysis detected 1.4- and 6.5-kb PLA2G12B transcripts in liver, kidney, skeletal muscle, and heart. Expression of PLA2G12B was significantly weaker in tumors of kidney, liver, and small intestine compared with matched normal tissues. PLA2G12B expression varied in cancer cell lines.

Alternative Names: PLA2G12B; FKSG71; GXIIB; GXIIIsPLA2; MGC138151; PLA2G13; group XIII secreted phospholipase A2; phospholipase A2; group XIII

Search name: PLA2G12B; FKSG71; GXIIB; GXIIIsPLA2; MGC138151; PLA2G13; group XIII secreted phospholipase A2; phospholipase A2; group XIII

Tag: PLA2G12B