Mouse GSTo1 (Glutathione S Transferase Omega 1) ELISA Kit | ELK3737

Mouse GSTo1 (Glutathione S Transferase Omega 1) ELISA Kit | ELK3737 | Gentaur UK, US & Europe Distribution

Alternative Names: P28; GSTTLp28; GSTw1; SPG-R; Glutathione-dependent dehydroascorbate reductase; Monomethylarsonic acid reductase; MMA (V) reductase; S- (Phenacyl) glutathione reductase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Omega 1 (GSTo1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione S Transferase Omega 1 (GSTo1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Omega 1 (GSTo1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Omega 1 (GSTo1) in the samples is then determined by comparing the OD of the samples to the standard curve.