Mouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit | E-EL-M0687

Mouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit | E-EL-M0687 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 75pg/mL

Detection Range: 125~8000pg/mL

UniProt ID: Q61221

Target Name: HIF-1α

Target Synonym: HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1α. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse HIF-1α. You can calculate the concentration of Mouse HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Mouse HIF-1α in samples. No significant cross-reactivity or interference between Mouse HIF-1α and analogues was observed.

Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.