Mouse High affinity cationic amino acid transporter 1 (SLC7A1) ELISA Kit | KTE70418

Mouse High affinity cationic amino acid transporter 1 (SLC7A1) ELISA Kit | KTE70418 | Gentaur UK, US & Europe Distribution

Application: This Mouse High affinity cationic amino acid transporter 1 (SLC7A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A1 in samples. An antibody specific for SLC7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse High affinity cationic amino acid transporter 1 microplate
• Mouse High affinity cationic amino acid transporter 1 standard
• Mouse High affinity cationic amino acid transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse High affinity cationic amino acid transporter 1 (SLC7A1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC7A1. No significant cross-reactivity or interference between Mouse SLC7A1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Susceptibility to murine ecotropic retroviruses is attributed to the binding of the virus envelope to the membrane receptor encoded by the Rec1 gene. HCAT1 was identified as the principal transporter of the cationic amino acids, arginine, lysine, and ornithine in mouse cells. the Rec1 gene mapped to mouse chromosome 5 by analysis of murine-hamster somatic cell hybrids. Using a human cDNA obtained by homology to Rec1, Albritton et al. (1992) determined the location of the human cationic amino acid transporter by somatic cell genetics, in situ hybridization, and RFLP linkage analysis. The studies indicated that ATRC1 is located at 13q12-q14, closely linked to ATP1AL1. The CEPH consortium linkage map of chromosome 13 published by Bowcock et al. (1993) showed ATRC1 to be distal to ATP1AL1.

Alternative Names: SLC7A1; RP11-274A8.1; ATRC1; CAT-1; ERR; HCAT1; REC1L; amino acid transporter; cationic 1; ecotropic retroviral receptor

Search name: SLC7A1; RP11-274A8.1; ATRC1; CAT-1; ERR; HCAT1; REC1L; amino acid transporter; cationic 1; ecotropic retroviral receptor

Tag: SLC7A1