Mouse Idian Hedgehog (IHH) ELISA Kit | KTE71386

Mouse Idian Hedgehog (IHH) ELISA Kit | KTE71386 | Gentaur UK, US & Europe Distribution

Application: This Mouse Idian Hedgehog (IHH) ELISA Kit employs a two-site sandwich ELISA to quantitate IHH in samples. An antibody specific for IHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Idian Hedgehog microplate
• Mouse Idian Hedgehog standard
• Mouse Idian Hedgehog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Idian Hedgehog (IHH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IHH. No significant cross-reactivity or interference between Mouse IHH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: IHH is one of three proteins in the mammalian hedgehog family, the others being desert hedgehog (DHH) and Sonic hedgehog (SHH) . It is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP) .Gao et al. (2001) decided to focus on IHH as the gene implicated in brachydactyly type A1 because of its position in the implicated interval on chromosome 2q35-q36 flanked by markers D2S2248 and D2S360 and becauseIHH is known to mediate condensation, growth, and differentiation of cartilage. They identified 3 heterozygous missense mutations in the region encoding the amino-terminal signaling domain in all affected members of 3 large, unrelated Chinese families.

Alternative Names: IHH; BDA1; HHG2; Indian hedgehog homolog

Search name: IHH; BDA1; HHG2; Indian hedgehog homolog

Tag: IHH