Mouse IDO2 (Indoleamine-2, 3-Dioxygenase 2) ELISA Kit | ELK6981

Mouse IDO2 (Indoleamine-2, 3-Dioxygenase 2) ELISA Kit | ELK6981 | Gentaur UK, US & Europe Distribution

Alternative Names: INDOL1; Indoleamine-Pyrrole 2, 3 Dioxygenase-Like 1; Indoleamine 2, 3-dioxygenase-like protein 1; Indoleamine-pyrrole 2, 3-dioxygenase-like protein 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2, 3-Dioxygenase 2 (IDO2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indoleamine-2, 3-Dioxygenase 2 (IDO2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2, 3-Dioxygenase 2 (IDO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indoleamine-2, 3-Dioxygenase 2 (IDO2) in the samples is then determined by comparing the OD of the samples to the standard curve.