Mouse IgM (Immunoglobulin M) ELISA Kit | ELK5383

Mouse IgM (Immunoglobulin M) ELISA Kit | ELK5383 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 54 ng/mL

Standard: 100000 ng/mL

Detection Range: 1562.5-100000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Infection immunity;Immune molecule;Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin M (IgM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin M (IgM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin M (IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.