Mouse IL-4 protein | PRP100498

Mouse IL-4 protein | PRP100498 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from mouse IL-4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.

Activity: Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-2 ng/mL. The ED50 for this effect is typically 0.4-2 ng/mL.

protein Lenght: The recombinant mouse IL-4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.

Preparation: E. coli

Purity: > 90 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 13.4 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Mouse IL-4 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.92% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Interleukin-4, also known as IL-4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL-4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL-4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL-4 receptor α, completely inhibites in a dose-dependent manner the IL-4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL-4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL-4-stimulated BA/F3 cells.

Alternative Names: BSF-1; Il-4; Interleukin-4; IL4

Search Names: BSF-1; Il-4; Interleukin-4; IL4

Tag: IL-4